In Silico Analysis and Expression of Influenza Virus 3M2e-HA2 Chimer Protein Fused to C-Terminal Domain of Leishmania major HSP70
نویسندگان
چکیده
منابع مشابه
بیان و خالص سازی پروتئین کایمر نوترکیب (3M2e-HA2) واجد نواحی حفاظت شده هماگلوتینین و پروتئین ماتریکس ویروس آنفلوانزا در راستای تولید واکسن زیرواحدی جهانی
Background and purpose: Influenza virus is one of the most important respiratory infectious agents. Viral antigenic variations are major problems for vaccine production process. At present, many researches have focused on conserved domains of influenza virus antigenic peptides for subunit vaccine development. Hemagglutinin small subunit (HA2) and the 23 amino acid extracellular N-terminal domai...
متن کاملIn Vitro Evaluation of Influenza M2 and Leishmania major HSP70 (221-604) Chimer Protein
BACKGROUND Permanent antigenic variation of influenza viruses causes a major concern to develop an effective human influenza vaccine. Conserved antigens are new vaccine candidates because it is not necessary to match the prepared vaccine with circulating strains. Ion channel M2 protein is conserved among all influenza A viruses, allowing the virus to enter host cells. OBJECTIVES To prepare an...
متن کاملin vitro evaluation of influenza m2 and leishmania major hsp70 (221-604) chimer protein
background permanent antigenic variation of influenza viruses causes a major concern to develop an effective human influenza vaccine. conserved antigens are new vaccine candidates because it is not necessary to match the prepared vaccine with circulating strains. ion channel m2 protein is conserved among all influenza a viruses, allowing the virus to enter host cells. objectives to prepare an e...
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The present study was aimed to construct a fusion plasmid harboring the extracellular domain of the influenza A M2-protein (M2e), which was fused to the N-terminus of the truncated HSP70 (HSP70359–610) molecule as a new approach for future vaccine research against influenza A. The amplified fragments, M2e and HSP70359-610 genes, were gel-purified. The products were then single digested with Bam...
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Antifungal agents are causing different problems in the agriculture industry. Plants are using various defense mechanisms for resistance against fungal pathogens. Some examples of these mechanisms are making physical barriers, producing chemical components and pathogenesis-related proteins such as lipid transfer protein (LTP) and Osmotin which can inhibit the growth of fungi at micro-molar conc...
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ژورنال
عنوان ژورنال: Jundishapur Journal of Microbiology
سال: 2018
ISSN: 2008-3645,2008-4161
DOI: 10.5812/jjm.13777